Click links to download protocols (pdf)
DNA sample collection: bacterioplankton
DNA sampling diagram using syringe
DNA sampling diagram using peristaltic pump
DNA extraction from sterivex filters
Bacterial Production as leucine incorporation rate: small volume for eutrophic systems
Bacterial Production as leucine incorporation rate: large volumes for oligotrophic systems
Dissolved phosphate measurement
Dissolved ammonium measurement
Click links to download diagrams of techniques for presentation. Please contact me (bcrump@hpl.umces.edu) if you would like the Adobe Illustrator versions.
Prokaryote cell counts
Leucine incorporation technique for measuring heterotrophic bacterial production
Respiration rate
Environmental Clone Library
Denaturing Gradient Gel Electrophoresis (DGGE) Terminal Restriction Fragment Length Polymorphism (TRFLP)
Automated Ribosomal Intergenic Spacer Analysis (ARISA)
Fluorescence In Situ Hybridization (FISH)
Fluorescence In Situ Hybridization plus microautoradiography (Micro-FISH)
Useful methods links
Denaturing Gradient Gel Electrophoresis DGGE guide from Stefan J. Green Detailed DGGE protocol from the University of Toledo Detailed DGGE protocol from Gabriel Zwart
ARB phylogenetics software ARB website ARB tutorial
Handy Links National Center for Biotechnology Information Ribosomal Database Project Unit Converter
