Methods
Click links to download protocols (pdf)
DNA sample collection: bacterioplankton
DNA sampling diagram using syringe
DNA sampling diagram using peristaltic pump
DNA extraction from sterivex filters
Bacterial Production as leucine incorporation rate: small volume for eutrophic systems
Bacterial Production as leucine incorporation rate: large volumes for oligotrophic systems
Dissolved phosphate measurement
Dissolved ammonium measurement
Useful methods links
Denaturing Gradient Gel Electrophoresis
DGGE guide from Stefan J. Green
Detailed DGGE protocol from the University of Toledo
Detailed DGGE protocol from Gabriel Zwart
ARB phylogenetics software
ARB website
ARB tutorial
Click links to download diagrams of techniques for presentation. Please contact me (bcrump@hpl.umces.edu) if you would like the Adobe Illustrator versions.
Leucine incorporation technique for measuring heterotrophic bacterial production
Denaturing Gradient Gel Electrophoresis (DGGE)
Terminal Restriction Fragment Length Polymorphism (TRFLP)
Automated Ribosomal Intergenic Spacer Analysis (ARISA)
Fluorescence In Situ Hybridization (FISH)
Fluorescence In Situ Hybridization plus microautoradiography (Micro-FISH)
Handy Links
National Center for Biotechnology Information
Ribosomal Database Project
Unit Converter